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graphpad prime 6.0  (GraphPad Software Inc)

 
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    GraphPad Software Inc graphpad prime 6.0
    Graphpad Prime 6.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/graphpad prime 6.0/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    graphpad prime 6.0 - by Bioz Stars, 2026-03
    90/100 stars

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    Size comparison of isolated extracellular vesicles from different cancer cell lines. Nanoparticle tracking analysis was performed to compare the sizes of EVs isolated from different cell lines. All cell lines were grown in RPMI medium with 10% FBS until log phase. Cell media were replaced with 10% exosome-free FBS with cells at a density of 0.5 × 10 6 ml –1 . After 48 h, the culture supernatants were filtered, PEG8000-precipitated, dialysed, and suspended in one-tenth of the original volume of PBS containing 10% glycerol. The resulting EV preparations were subjected to nanoparticle tracking analysis. Dot plot were graphed with statistical software GraphPad Prime version 6.0 from five readings of samples prepared by ultracentrifugation (Ↄ) or PEG precipitation (□) side by side from different cell lines. CHLA-255, CHLA-136, SK-N-BE2, IMR-32 are neuroblastoma cell lines; H2228 is a non-small cell lung cancer adenocarcinoma cell line; HEK293 is a kidney embryonic cell line; and IMR-90 cells are normal fibroblasts.
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    Size comparison of isolated extracellular vesicles from different cancer cell lines. Nanoparticle tracking analysis was performed to compare the sizes of EVs isolated from different cell lines. All cell lines were grown in RPMI medium with 10% FBS until log phase. Cell media were replaced with 10% exosome-free FBS with cells at a density of 0.5 × 10 6 ml –1 . After 48 h, the culture supernatants were filtered, PEG8000-precipitated, dialysed, and suspended in one-tenth of the original volume of PBS containing 10% glycerol. The resulting EV preparations were subjected to nanoparticle tracking analysis. Dot plot were graphed with statistical software GraphPad Prime version 6.0 from five readings of samples prepared by ultracentrifugation (Ↄ) or PEG precipitation (□) side by side from different cell lines. CHLA-255, CHLA-136, SK-N-BE2, IMR-32 are neuroblastoma cell lines; H2228 is a non-small cell lung cancer adenocarcinoma cell line; HEK293 is a kidney embryonic cell line; and IMR-90 cells are normal fibroblasts.

    Journal: Journal of Extracellular Vesicles

    Article Title: Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells

    doi: 10.1080/20013078.2017.1294368

    Figure Lengend Snippet: Size comparison of isolated extracellular vesicles from different cancer cell lines. Nanoparticle tracking analysis was performed to compare the sizes of EVs isolated from different cell lines. All cell lines were grown in RPMI medium with 10% FBS until log phase. Cell media were replaced with 10% exosome-free FBS with cells at a density of 0.5 × 10 6 ml –1 . After 48 h, the culture supernatants were filtered, PEG8000-precipitated, dialysed, and suspended in one-tenth of the original volume of PBS containing 10% glycerol. The resulting EV preparations were subjected to nanoparticle tracking analysis. Dot plot were graphed with statistical software GraphPad Prime version 6.0 from five readings of samples prepared by ultracentrifugation (Ↄ) or PEG precipitation (□) side by side from different cell lines. CHLA-255, CHLA-136, SK-N-BE2, IMR-32 are neuroblastoma cell lines; H2228 is a non-small cell lung cancer adenocarcinoma cell line; HEK293 is a kidney embryonic cell line; and IMR-90 cells are normal fibroblasts.

    Article Snippet: Dot plot were graphed with statistical software GraphPad Prime version 6.0 from five readings of samples prepared by ultracentrifugation (Ↄ) or PEG precipitation (□) side by side from different cell lines.

    Techniques: Isolation, Software